Diagnostic singleplex PCR

This technique is based on the use of species specific molecular markers. Species specific molecular probes are developed, that only bind on the template DNA when a given sample belongs to a certain species. Depending on you research question, these molecular probes can help to differentiate various taxonomic levels (species, genus, family). In these cases, a higher resolution requires further analysis. A comprehensive evaluation of our diagnostic singleplex assays regarding their specificity and sensitivity is guaranteed. This approach is ideally suited for the selective search of specific species, such as endangered, invasive or protected species, especially when sample numbers are high.

  1. A sample containing a mixture of different species (e.g. in faeces, gut contents, etc.) or bulked samples are the basis of the analysis.
  2. DNA of all organisms in your sample are extracted.
  3. Detection of DNA from  a certain target species via specifically developed, highly sensitive molecular probes.
  4. Analysis of the presence of the target species in your sample (present/absent).
  5. Concentration of target DNA can be determined semi-quantitatively and quantatively upon your request.

 

What you get:

We will be happy to provide you with anything else besides that. So, if you need further analysis, please contact us.

What we need:

Your personal requirements may vary according upon your specific needs. For details please contact us.

Diagnostic multiplex PCR assays

Diagnostic multiplex PCR represents an extension of the diagnostic singleplex PCR. The combination of multiple molecular probes allows the parallel detection of multiple taxa within a single reaction. The number of target species is limited, but the combination of numerous multiplex assays can lead to a higher resolution. The simultaneous detection of multiple taxa within a single reaction makes this approach highly efficient, thus enabling the identification of target speices at high sensitivity and specificity also in complex samples (water, eDNA, faeces, gut content etc.).

  1. A sample containing a mixture of different species (e.g. in faeces, gut contents, etc.) or bulked samples are the basis of the analysis.
  2. DNA of all organisms in your sample are extracted.
  3. Detection of DNA from  a number of certain target species via specifically developed, highly sensitive molecular probes.
  4. Analysis of the presence of the different target species in your sample (present/absent).
  5. Concentration of target DNA can be determined semi-quantitatively and quantatively upon your request

 

What you get:

We will be happy to provide you with anything else besides that. So, if you need further analysis, please contact us.

What we need:

Your personal requirements may vary according upon your specific needs. For details please contact us.