Spectrophotometric DNA Quantification
For rapid and accurate DNA quantification of DNA extracts we use NanoDrop™ spectrophotometers (Thermo Scientific™). Only 1–2µL of the sample, pipetted directly onto an optical measurement surface, are sufficient for the assessment of DNA concentrations and potential contaminations.
Droplet digital™ PCR
This represents a new, automated method for DNA quantification during PCR. Compared to conventional methods like qPCR the ddPCR provide several advantages, such as that it does not imply standard curves any more. It is especially useful for low DNA concentrations and provides robust and reliable results.
The relationship of samples, where the target species has/have been detected to the number of samples where it/they has/have been absent provide an estimate of the abundance of a given species.