Spectrophotometric DNA Quantification
For rapid and accurate DNA quantification of DNA extracts we use NanoDrop™ spectrophotometers (Thermo Scientific™). Only 1–2µL of the sample, pipetted directly onto an optical measurement surface, are sufficient for the assessment of DNA concentrations and potential contaminations.
Droplet digital™ PCR
This represents a new, automated method for DNA quantification during PCR. Compared to conventional methods like qPCR the ddPCR provide several advantages, such as that it does not imply standard curves any more. It is especially useful for low DNA concentrations and provides robust and reliable results.
Quantitative PCR (qPCR)
This method, also known as real-time PCR (RT PCR), is a technique where the amplification of DNA molecules during PCR is monitored and quantfied. The measurement of the increase of DNA molecules takes place via fluorescence detection in the exponential phase of the PCR, the time when optimal reaction conditions are present. The quantification is done via comparison to internal controls.
The relationship of samples, where the target species has/have been detected to the number of samples where it/they has/have been absent provide an estimate of the abundance of a given species.